中英
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double-stranded dna中文是什么意思
- 中文翻譯
- 造句
- 雙鏈dna
- double adj. 1.兩倍的，加倍的。 2.雙的，二重的，雙重的 ...
- strand n. 1.(繩子的)股，絞；一股繩子；纖維，繩，線；串。 ...
- dna DNA ＝ 1.deoxyribonucle ...
- antibodies to double stranded dna 雙螺旋脫氧核糖核酸抗體
- circular double stranded dna 環(huán)狀雙鏈dna
- circular double-stranded dna 環(huán)狀雙股脫氧核糖核酸
- double stranded dna 雙鏈脫氧核糖核酸,雙鏈dna
- double-stranded cyclic dna 雙鏈環(huán)狀dna
- double-stranded dna virus 雙鏈dna簿
- double-stranded linear dna 雙鏈線性dna
- anti-double-stranded dna antibody 抗雙鏈dna抗體
- double stranded anti dna antibody 雙股抗dna抗體
- histone-free double stranded dna 無組蛋白雙鏈dna
- anti-double-stranded dna antibody test 抗雙鏈dna抗體試驗,抗dsdna抗體試驗
- double stranded 雙鏈的
- double-stranded 雙鏈,雙股,雙鏈的,雙股的; 以鏈的
- circular single stranded dna 環(huán)狀單股脫氧核糖核酸
- circular single-stranded dna 環(huán)狀單股脫氧核糖核酸
- multicopy single-stranded dna 多烤貝單鏈dna
- single stranded dna 單鏈dna
- single-stranded cyclic dna 環(huán)狀單鏈dna,環(huán)狀單股dna
- single-stranded dna 單鏈、單股dna,單鏈脫氧核糖核酸,單股dna,單鏈dna
- single-stranded dna virus 單股dna病毒; 單股dna簿
- single-stranded dna viruses 單鏈dna病毒; 單鏈dna簿
- single-stranded linear dna 直線單鏈dna
- The cyanophage contained a double - stranded dna genome at ca . 36 . 6kb
病毒的遺傳物質(zhì)為雙鏈dna ，基因組的大小約36 . 6kb 。 - The genetic material of the new virus is arranged in a similar fashion , encoded in circular , double - stranded dna , and the virus ' s five proteins have similarities to the proteins of other polyoma viruses
新病毒的遺傳物質(zhì)排列方式與多瘤病毒類似，編碼為環(huán)狀的雙鏈dna ，新病毒的五個蛋白質(zhì)與其他多瘤病毒有相似之處。 - To investigate the silence effect of hela cells " telomerase gene expression after shrna based on human telomerase htert transfected into cells . methods : we constructed a partial double - strand dna with t7 promoter as dna template and synthesized small hairpinrna in - vitro using t7 rna polymerase
方法：根據(jù)端粒酶htert基因1573 ? 1591位的核酸序列，構(gòu)建帶t7啟動子的部分雙鏈dna模板，用t7rna聚合酶體外合成短鏈shrna 。 - Conclusions : the in - vitro method that partial double - strand dna with t7 wi l . - toi promoter sequence as template and synthesizing by t7 rna polymerase can product high yield , excellent purity shrna . lt is a convenient - , effective ^ low - cost method and fit for small rna synthesis and rna interference researches in ordinary laboratory
結(jié)論：以帶t7啟動子部分雙鏈dna為模板，用t7rna聚合酶體外合成出的shrna產(chǎn)量較高，純度較好，是一種簡便、高效、低成本的短鏈rna的制備方法，適合于普通實驗室用來進行短鏈rna的合成和rna干擾實驗。 - The mechanism is that the introduced complementary oligonucleotides can bind to the corresponding mrna or double - stranded dna in genome and form partial double - stranded molecules or triple - stranded nucleic acid molecules by sequence - specific and nonsequence - specific antisense action , thus the target gene will be orientationally blocked and expression of the target inhibited so that therapeutic effect could be attained . in this study , we designed a fragment of human c ii ta cdna in antisense orientation using mrna of c ii ta as template . the primers were designed based on 94 - 500 nucleotides segment in 5 " end of ciita gene so that the interested gene contained 407 base pairs which included two aug codons in 1 16 and 188 nucleotides as well as the splicing site between the first and the second exons
本研究設(shè)計以c tamrna為模板的反義cdna片段，從c ta基因5 ’端第94位到500位核苷酸段設(shè)計引物，目的片段407bp ，覆蓋第116和188位兩個aug密碼子，也包含了第一外顯子和第二外顯子間的剪接位點：用常規(guī)分子生物學(xué)方法構(gòu)建了反義片段的腺病毒表達載體( padeasy - 1系統(tǒng)) ；腺病毒載體經(jīng)hek293細胞包裝產(chǎn)生含反義片段的重組腺病毒，用氯化銫密度梯度離心法獲得純化的高滴度腺病毒；進行體外基因轉(zhuǎn)移，分別用反義片段真核表達載體轉(zhuǎn)染p388d1細胞和用重組腺病毒感染hela細胞，觀察導(dǎo)入的c ta基因反義rna抑制細胞內(nèi)組成型或誘導(dǎo)型c ta基因表達的作用，從而達到調(diào)控mhc -類分子表達的目的。 - In this study , the suitable parameters for the introduction of plasmids phz1358 and pset152 into s . nanchangensis ns3226 from e . coli were tested for developing an conjugation system for s . nanchangensis ns3226 . a dnd gene cluster , which encodes an unknown modification system for 5 hvidans 1326 and renders its dna susceptible to site - specific double - strand dna cleavage during electrophoresis was conjugated from e . coli into s . nanchangensis ns3226
將克隆在整合型載體pset152上的變鉛青鏈霉菌1326的dnd基因簇通過接合轉(zhuǎn)移導(dǎo)入野生型南昌鏈霉菌ns3226中進行異源表達，觀察到接合轉(zhuǎn)移子的dna獲得了在含fe ~ ( 2 + )的電泳緩沖液中電泳時降解的表型。
- double-stranded dnaとは意味：《生化學(xué)》二本鎖｛にほんさ｝DNA
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